Clone

Target
IDP05488  
Clone Suffix
1
Experiment Start Date
Oct 20, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
237  
Reference Sequence Used
MKKVSVLPAF IITFVCMLAF LVMPYGNASA KLADGTYDIN YVIQKAENDS ASMANDYFEK PAKLIVKNGE MRVQVPMNHS AWITEFKAPE NGNFVDAKVV SKDESADKRT VEFKVDDLSK PAAVKIHVVV PNANYDHHYT IRFAFDANVK AVGGDNGVAA TTKNNDQAKT DTQVKEEKTK VESKETAKEV NKETKNENGK AEKTDNPKTG DEARIGLFAA LILISGVFLI RKVKLSK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAAAGGTTTCTGTATTACC  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTACTCAATTTCACTTTACG  

Related Expressions

         
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2014-02-19 2014-02-20 Karol Szlachta University of Virginia uva_induction_1 success Ecoli LB 0.25 mM IPTG production
2015-06-09 2015-06-11 Jing Hou University of Virginia uva_expression_native_1 success E. coli LB IPTG production
2016-03-07 2016-03-10 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB with 1mM FeCl2 after induction IPTG production
2016-03-16 2016-03-18 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB IPTG production
2016-03-16 2016-03-18 Jing Hou University of Virginia uva_expression_native_1 success E.coli LB with FeCl2 IPTG production