Clone

Target
IDP05597  
Clone Suffix
1
Experiment Start Date
Oct 20, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
199  
Reference Sequence Used
MLKKLIVGAV VAISSLTVGL GSVSASTEKD YSITSFEYVT VDEKTVDSFN KLGAPSKEDI KITLVLPKQN QNGDWLSYGF SSKESLESFI EKDKQRLNHT ITPLGVEGSG PGSTDFYKRT NQGSEYFSLS SGYKNLSPSW QNQISSVSTA SPTASYSTTL WERTSTEGYG KGVVFKHADW YGKTANLAAI PTSAVEVKK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGCTTAAAAAATTAATAGTAGGTG  
PCR Backward Primer
TTATCCACTTCCAATGTTACTTTTTAACTTCAACTGCTG  
Shipped To Lab
ANL  
Shipped Date
May 11, 2011  
Secondary Shipped To Lab
UT  
Secondary Shipped Date
Sep 19, 2011  
Inserted
Nov 24, 2010  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-04-26 2011-04-26 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2012-03-07 Maite Garcia University of Calgary Expression_protocol_Toronto success E. coli 15N 2X M9 none production