Clone

Target
IDP05715  
Clone Suffix
1
Experiment Start Date
Oct 20, 2010  
Experiment End Date
Nov 08, 2010  
Person
Sarah Grimshaw  
Lab
J. Craig Venter Institute  
Protocol ID
CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
305  
Reference Sequence Used
MKKLFISLTV LFVLVMSACS NGSTDKKNDA KGSKSETITY QSENGKVEVP ANPKRVVVLS SFAGNVMSLG VNLVGVDSWS KQNPRFDSKL KDVAEVSDEN VEKIAELNPD LIIGLSNIKN VDKLKKIAPT VTYTYGKVDY LTQHLEIGKL LNKEKEAKTW VDDFKKRAQD AGKEIKAKIG EDATVSVVEN FNKQLYVYGE NWGRGTEILY QEMKLKMPEK VKEKALKEGY YALSTEVLPE FAGDYLIVSK NKDTDNSFQE TESYKNIPAV KNNRVYEANM MEFYFNDPLT LDFQLDFFKK SFLGK  
Reference Sequence Variance
MATCH  
PCR Forward Primer
TACTTCCAATCCAATGCGATGAAAAAGTTATTTATTTCACTAAC  
PCR Backward Primer
TTATCCACTTCCAATGTTATTTACCAAGAAAGCTCTTCT  

Related Expressions

       
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2010-11-17 2010-11-17 Sarah Grimshaw J. Craig Venter Institute CSG-004_Transformation, Frozen Culture and Overexpression of Recombinant Proteins success E. coli 2xYT 1mM IPTG assay
2011-08-02 2011-08-05 Rob Solberg University of Virginia uva_expression_semet_1 success E. coli SeMet IPTG production
2014-02-19 2014-02-20 Karol Szlachta University of Virginia uva_expression_native_1 success Ecoli LB 0.25 mM IPTG production
2014-02-24 2014-02-24 Karol Szlachta University of Virginia allergens_purification_1 success Ecoli LB 0.5 mM IPTG production