Clone

Target

IDP00567  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

935  

Reference Sequence Used

MQNGAMKAWL DSSYLAGANQ SYIEQLYEDF LTDPGSVDDS WRSIFQQLPT TGVKPDQLHS QTREYFRRLA KDSTRYNSSI NDPDIDAKQV KVLQLINAFR FRGHQHANLD PLGLWKQESV PDLDPSYHNL TEADFQNTFN VGSFAIGKET MKLADLYAAL KQTYCGSVGA EYMHITNTEE KRWIQQRIES VVGKPTFSDA EKRRFLSELT AAEGLERYLG AKFPGAKRFS LEGGDSLVTM LKEMIRHAGK NGTREVVLGM AHRGRLNVLI NVLGKKPEDL FDEFAGKHKE HLGTGDVKYH QGFSSDVETE GGLVHLALAF NPSHLEIVSP VVIGSVRARR DRLDEARSNM VLPITIHGDA AITGQGVVQE TLNMSQARGY EVGGTVRIVI NNQIGFTTSN PLDARSTQYC TDIAKMVQAP IFHVNADDPE AVAFVTRLAL DFRNTFKRDV MIDLVCYRRH GHNEADEPSA TQPVMYQKIK KHPTPRKIYA DKLIEQNIAS LEDATEMVNL YRDALDHGDC VVEEWRPMNL QSFTWSPYLN HEWDEAYPSK VEMKRLQELA RRISSAPEAI EMQSRVAKIY SDRALMASGE KPFDWGGAET LAYATLVDEG IPIRLSGEDA GRGTFFHRHA VIHNQKNGSV YVPLSNIHSG QGDFQVWDSV LSEEAVLAFE YGYATAEPRT LTIWEAQFGD FANGAQVVID QFISSGEQKW GRMCGLVMLL PHGYEGQGPE HSSARLERYL QLCAEQNMQV CIPSTPAQVY HMIRRQALRG MRRPLIVMSP KSLLRHPLAT SSLDELANGS FLPAIGEIDE LDPKGIKRVV MCSGKVYYDL LEQRRKNGQT DVAIVRIEQL YPFPHQAVQS VLEQYAHVHD FVWCQEEPLN QGAWYCSQHN FREVIPFGAS LRYAGRPASA SPAVGYLSVH QKQQQALVND ALNVE  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCAGAACGGCGCAATGAA  

PCR Backward Primer

TTATCCACTTCCAATGCTATTCAACATTCAGCGCGTC  

Shipped To Lab

ANL  

Shipped Date

Jul 16, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008