Clone

Target
IDP00108  
Clone Suffix
103
Sequence Comments
IDP00108.1-309.F..pMCSG7  
Experiment Start Date
Jan 10, 2008  
Experiment End Date
Jan 10, 2008  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol ID
standard cloning_ANL  
Status
success  
Vector
pMCSG7  
Sequence Start
1  
Sequence End
309  
Reference Sequence Used
MSIRIIPQDE LGSSEKRTAD MIPPLLFPRL KNLYNRRAER LRELAENNPL GDYLRFAALI AHAQKVVLYD HPLEMDLTAR IKEASAQGKP PLDIHVLPRD KHWQKLLMAL IAELKPEMSG PALAVIENLE KASTQELEDM ASALFASDFS SVSSDKAPFI WAALSLYWAQ MANLIPGKAR AEYGEQRQYC PVCGSMPVSS MVQIGTTQGL RYLHCNLCET EWHVVRVKCS NCEQSGKLHY WSLDDEQAAI KAESCDDCGT YLKILYQEKE PKVEAVADDL ASLVLDARME QEGYARSSIN PFLFPGEGE  
PCR Forward Primer
TACTTCCAATCCAATGCCATGAGTATTCGCATAATCCCGCAAGATG  
PCR Backward Primer
TTATCCACTTCCAATGTTACTCTCCTTCACCCGGAAACAG  
Shipped To Lab
ANL  
Shipped Date
Jan 10, 2008  
Inserted
Jul 29, 2011  

Related Expressions

     
Experiment
Date Start		 Experiment
Date End		 Person Lab Protocol Status Expression
Organism		 Media
Type		 Induction
Reagent		 Experiment
Type
2008-01-21 2008-01-24 Minyi Gu Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG assay
2008-07-10 2008-07-20 Min Zhou Argonne National Laboratory standard expression_ANL failed E. coli M9 minimal + semet 1 mM IPTG production
2008-07-10 2008-07-20 Min Zhou Argonne National Laboratory standard expression_ANL success E. coli M9 minimal 1 mM IPTG production