Clone

Target

IDP00516  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

317  

Reference Sequence Used

MRLRLALFSS LLAATFAITG CDQSSETPDN AATISIEHAQ GTTLVPLNPQ KVVILNPSVL DNADALHIKV AGVPQTSTHL PAFLSKYSGP EYMNTGTLFE PDYEALSQAK PDLIIAGGRA QDAYNKLSAI APTIALDVDT QHFTQSLTQR TEQLASIFGK EEEAKTLLGN FSSQVNAIKQ KSANAGSAMV LMISGGKMSA YTPGSRFGFI FDELGFTPAA TFAESGRHGN VVTSEFILNA NPDWLFVLDR DNAIGRTEGQ SAQQVLDNPL IHKTKAWQND RIVYLDSASL YIAGGVQSYT QLMNKVSAIL DKPVSEQ  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGCGATTACGTTTAGCTTTATT  

PCR Backward Primer

TTATCCACTTCCAATGTTACTGCTCTGATACTGGCTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008