Protocols

Display Protocols
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ShortNameLabTypeDescription
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1717UTcloningpMCSG7
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1818UTcloningEscherichia coli DH5a
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1919UTcloningInsertion of a DNA sequence into the cloning/expression region is performed using Clontech¿s In-fusion enzyme-mediated directional recombination between complementary 15 nucleotide DNA sequences at t ...
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2020UTcloningTo be used as a ligation independent cloning vector, 15 bp addition to 5¿end of primers Fwd Primer: 5¿-GGA GAT ATA CAT ATG-----3¿ Rev Primer: 5¿-GTG GTG GTG CTC GAG-----3¿ Remove start a ...
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201201UTcloning
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2323UTcloningTDB
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2525UTcloningThe pFHMSP-LIC N vector is a derivative of the pFastBac HT A vector (Invitrogen). Honeybee melittin signal sequence was introduced before His tag. It is a donor vector for generation of recombinant ba ...
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2929UTcloningToronto cloning protocol. Description is coming.
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44UTcloningpET28b
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66UTcloningpET28a-LIC To be used as a ligation independent cloning vector, 15bp addition to 5'end of primers Fwd Primer: 5'-gtt ccg cgt ggt agt -----3' Rev Primer: 5'-caa gct tcg tca tca -----3' Contains 2 ...
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