Protocol

Abbreviation

NU_default_crystallization

Name

NU robot crystallization

Laboratory name

Northwestern University

Type

crystallization

Description

Crystallization experiments are usually performed by vapor diffusion method using Phoenix RE crystallization robot with 96-DWBlock screens from Qiagen and sitting drop 96-well crystallization plates. Every reservoir well contains 100ul of screen solution and every sample well- 1ul of protein (concentrations 7-10mg/ml) mixed with 1ul of reservoir solution.