Center for Structural Genomics of Infectious Diseases

Purifications

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Page 10 of 820, showing 20 records out of 16392 total, starting on record 181, ending on 200

Id	Target	Purification Suffix	Expression	Local Expression	Local Protein Target	Local Purification	Experiment Date Start	Experiment Date End	Staff	Lab	Protocol	Status	Description	Affinity Tag Status	Semet Status	Final Buffer	Final Concentration	Final Volume	Shipped To Lab	Shipped Date	Actions
7594	IDP00025	3079	12916	1069	LAXHqZcPyV5zPDS6dzeQGbMYvsw	3079	Sep 10, 2008	Sep 12, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-09-12	View Edit Delete
7595	IDP00025	3078	12916	1069	LAXHqZcPyV5zPDS6dzeQGbMYvsw	3078	Sep 10, 2008	Sep 12, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-09-12	View Edit Delete
7589	IDP00025	4181	12916	1069	LAXHqZcPyV5zPDS6dzeQGbMYvsw	4181	Sep 10, 2008	Sep 12, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-09-12	View Edit Delete
7593	IDP00025	3080	12916	1069	LAXHqZcPyV5zPDS6dzeQGbMYvsw	3080	Sep 10, 2008	Sep 12, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-09-12	View Edit Delete
9604	IDP00026	64097	16139				Feb 28, 2012		Maite Garcia	UT	Purification_protocol_Toronto	failed		no his-tag protein eluted	native		0	0			View Edit Delete
8383	IDP00027	3581	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3581	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	1	18000	ANL	2008-09-12	View Edit Delete
8384	IDP00027	3879	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3879	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	1	18000	ANL	2008-09-12	View Edit Delete
8385	IDP00027	3877	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3877	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	1	18000	ANL	2008-09-12	View Edit Delete
5957	IDP00027	3878	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3878	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::SEC::SEC peak 1	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	33	850	ANL	2008-09-12	View Edit Delete
5774	IDP00027	3580	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3580	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	1	18000	ANL	2008-09-12	View Edit Delete
5956	IDP00027	3880	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3880	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	33	850	ANL	2008-09-12	View Edit Delete
5958	IDP00027	3582	12917	1070	gVWvrFpLmvFS0w6NaY5Fe5Wiy5c	3582	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	33	850	ANL	2008-09-12	View Edit Delete
7596	IDP00028	3884	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3884	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7597	IDP00028	3883	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3883	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7598	IDP00028	3882	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3882	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::SEC::SEC peak 1	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7599	IDP00028	3881	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3881	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7600	IDP00028	3585	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3585	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7601	IDP00028	3584	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3584	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-II program::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7602	IDP00028	3583	12918	1071	Lp+c+d/eJsJxxroKcSDFcyLh0fw	3583	Sep 10, 2008	Sep 12, 2008	Minyi Gu	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress using IMAC-I program::ÃKTAxpress IMAC-I program eluate (250 mM imidazole)	cleaved	semet	20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT	0	0	ANL	2008-09-12	View Edit Delete
7547	IDP00029	3083	12893	1072	dPkedUtcqmHSjaEjsvcu9jiZpgI	3083	Sep 10, 2008	Sep 12, 2008	Min Zhou	ANL	standard purification_ANL	success	IMAC-I, SEC, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)	na	semet	50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole	0	0	ANL	2008-09-12	View Edit Delete

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