Purification

Target
IDP00038  
Purification ID
A11955  
Expression
LB (Sep 10, 2012)  
Experiment Date Start
Sep 10, 2012  
Experiment Date End
Sep 12, 2012  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
7  
Final Volume (μL)
5000  
Shipped To Lab Id
ANL  
Shipped Date
Sep 12, 2012  
Inserted
Nov 01, 2012