Purification

Target
IDP00038  
Purification ID
A11956  
Expression
LB (Sep 10, 2012)  
Experiment Date Start
Sep 10, 2012  
Experiment Date End
Sep 12, 2012  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
70  
Final Volume (μL)
500  
Shipped To Lab Id
ANL  
Shipped Date
Sep 12, 2012  
Inserted
Nov 01, 2012