Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP92620
Purification ID: V744
Expression: LB (Jul 03, 2012)
Experiment Date Start: Sep 23, 2013
Experiment Date End: Sep 25, 2013
Person: Jing Hou
Lab: University of Virginia
Protocol: allergens_purification_1
Status: success
Description
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer
Final Concentration (mg/mL): 0
Final Volume (μL): 0
Shipped To Lab Id
Shipped Date
Inserted: Oct 07, 2013

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	pH	Plate Type
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Sodium Chloride, 0.01M Dithioerythritol, 0.1M Bis-Tris, 25%w/v PEG 3350	5.5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1M Bis-tris propane, 0.2Molal Ammonium Sulfate, 0.01M Dithioerythritol, 30%w/v PEG 3350	6	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	35%w/v PEG 3350, 0.1M Bis-Tris, 0.01M Dithioerythritol	5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.01M Dithioerythritol, 0.1M Bis-Tris, 20%w/v PEG 3350	5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.01M Dithioerythritol, 0.1M Bis-Tris, 40%w/v PEG 3350	5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%w/v PEG 3350, 0.1M Bis-Tris, 0.01M Dithioerythritol, 0.2M Sodium Chloride	5.5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	25%w/v PEG 3350, 0.01M Dithioerythritol, 0.1M Bis-tris propane, 0.2Molal Ammonium Sulfate	6	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	30%w/v PEG 3350, 0.1M Bis-Tris, 0.2M Sodium Chloride, 0.01M Dithioerythritol	5.5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.01M Dithioerythritol, 0.1M Bis-Tris, 0.2M Sodium Chloride, 35%w/v PEG 3350	5.5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	40%w/v PEG 3350, 0.2M Sodium Chloride, 0.1M Bis-Tris, 0.01M Dithioerythritol	5.5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Ammonium Sulfate, 0.1M Bis-tris propane, 0.01M Dithioerythritol, 20%w/v PEG 3350	6	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	25%w/v PEG 3350, 0.01M Dithioerythritol, 0.1M Bis-Tris	5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	35%w/v PEG 3350, 0.01M Dithioerythritol, 0.2Molal Ammonium Sulfate, 0.1M Bis-tris propane	6	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.01M Dithioerythritol, 0.1M Bis-Tris, 30%w/v PEG 3350	5	QIAGEN EasyXtal 15-Well Tool
Oct 07, 2013	Jing Hou	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.01M Dithioerythritol, 0.2Molal Ammonium Sulfate, 0.1M Bis-tris propane, 40%w/v PEG 3350	6	QIAGEN EasyXtal 15-Well Tool