Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00044
Purification ID: V303
Expression: SeMet (May 10, 2009)
Experiment Date Start: May 11, 2009
Experiment Date End: May 12, 2009
Person: Majka Klimecka
Lab: University of Virginia
Protocol: uva_expression_semet_1
Status: success
Description
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer
Final Concentration (mg/mL): 11
Final Volume (μL): 1200
Shipped To Lab Id
Shipped Date
Inserted: May 19, 2009

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	pH	Plate Type
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 15%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 22%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 22%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 22%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 15%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	20%v/v PEG 3350, 0.18M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	22%v/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 15%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	17%v/v PEG 3350, 0.14M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%v/v PEG 3350, 0.12M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool
Jul 11, 2016	Mateusz Czub	University of Virginia	UVA_default_crystallization	nothing	sitting drop	10%v/v PEG 3350, 0.08M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 30%v/v Ethylene glycol	7.5	QIAGEN EasyXtal 15-Well Tool