Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP92725
Purification ID: A16375
Expression: LB (Oct 28, 2013)
Experiment Date Start: Nov 04, 2013
Experiment Date End: Nov 06, 2013
Person: Boguslaw Nocek
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: PIG-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 60
Final Volume (μL): 600
Shipped To Lab Id: ANL
Shipped Date: Nov 06, 2013
Inserted: Dec 13, 2013

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	Screen	Screen Component	pH	Plate Type
Nov 25, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop		Wizard Full (I & II)	62		CrystalQuick standard profile, LBR round bottom plate
Nov 25, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop		Index Screen	51		CrystalQuick standard profile, LBR round bottom plate
Nov 25, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop		Index Screen	89		CrystalQuick standard profile, LBR round bottom plate
Dec 01, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	hanging drop	Peg3350	Index Screen	5
Dec 01, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	hanging drop	Magnesium Chloride	Wizard Full (I & II)	65
Dec 01, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	hanging drop	2-Methyl-2,4-pentanediol	Index Screen	53
Dec 03, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	hanging drop		Index Screen	81	8.5
Nov 25, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop		Index Screen	81		CrystalQuick standard profile, LBR round bottom plate
Nov 25, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop		Index Screen	76		CrystalQuick standard profile, LBR round bottom plate
Nov 25, 2013	Boguslaw Nocek	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop		Index Screen	79		CrystalQuick standard profile, LBR round bottom plate