Purification

Target
IDP00258  
Purification ID
W1  
Expression
Athena_SeMET (Sep 10, 2008)  
Experiment Date Start
Sep 15, 2008  
Experiment Date End
Sep 17, 2008  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_inclusion_body_expression_2  
Status
success  
Description
Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.  
Affinity Tag Status
uncleaved  
SeMet Status
semet  
Final Buffer
25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
7.5  
Final Volume (μL)
200  
Shipped To Lab Id
WU  
Shipped Date
Sep 17, 2008  
Inserted
Oct 02, 2008