Purification

Target
IDP00260  
Purification ID
W1  
Expression
2xYT (Apr 29, 2009)  
Experiment Date Start
Aug 25, 2008  
Experiment Date End
Aug 26, 2008  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_inclusion_body_expression_2  
Status
success  
Description
Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. 4 hours denaturation at room temp. Works in co-refolding NKG2D.  
Affinity Tag Status
uncleaved  
SeMet Status
semet  
Final Buffer
25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
14  
Final Volume (μL)
300  
Inserted
Oct 02, 2008