Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00272
Purification ID: W1
Expression: 2xYT (Aug 03, 2009)
Experiment Date Start: Feb 25, 2010
Experiment Date End: Feb 26, 2010
Person: Chris Nelson
Lab: Washington University
Protocol: WU_native_inclusion_body_expression_2
Status: success
Description: Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column. 4 hours denaturation at room temp.
Affinity Tag Status: uncleaved
SeMet Status: semet
Covalent Modification
Final Buffer: 25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide
Final Concentration (mg/mL): 12
Final Volume (μL): 200
Shipped To Lab Id
Shipped Date
Inserted: Oct 02, 2008

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	Screen	pH	Plate Type
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	small crystal	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 12.5 ul Glycerol	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	2 M Ammonium Sulfate, 0.2 M Na Citrate pH5.5, 0.025 M Taurine	add new		Hampton_24_well
Dec 02, 2012	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	PEG 6,000, and 0.1 M MES pH 5.0	Optimization screen	5.7