Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00272
Purification ID: W2
Expression: LB (Jan 28, 2008)
Experiment Date Start: Feb 04, 2008
Experiment Date End: Feb 05, 2008
Person: Chris Nelson
Lab: Washington University
Protocol: WU_native_inclusion_body_expression_2
Status: success
Description: Arginine refolding, followed by gel filtration on Superdex 200 16/60 HR column.
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide
Final Concentration (mg/mL): 14
Final Volume (μL): 400
Shipped To Lab Id
Shipped Date
Inserted: Oct 02, 2008

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	Screen	pH	Plate Type
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.150 M, PEG 3000 32%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.150 M, PEG 3000 31%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.175 M, PEG 3000 28%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.175 M, PEG 3000 29%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.175 M, PEG 3000 30%	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH8.0, 0.2 M Lithium Sulfate, 32% PEG 3000	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.150 M, PEG 3000 29%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.200 M, PEG 3000 28%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.200 M, PEG 3000 29%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.200 M, PEG 3000 31%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.200 M, PEG 3000 32%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.200 M, PEG 3000 33%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.225 M, PEG 3000 28%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.225 M, PEG 3000 29%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.225 M, PEG 3000 30%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.225 M, PEG 3000 31%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.225 M, PEG 3000 32%	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.5, 0.2 M Lithium Sulfate, 32% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.5, 0.2 M Lithium Sulfate, 33% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.75, 0.2 M Lithium Sulfate, 30% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.75, 0.2 M Lithium Sulfate, 28% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.75, 0.2 M Lithium Sulfate, 29% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.75, 0.2 M Lithium Sulfate, 31% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.75, 0.2 M Lithium Sulfate, 32% PEG 3000	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.150 M, PEG 3000 28%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.225 M, PEG 3000 33%	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.3, 0.2M Lithium Sulfate, 30% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.3, 0.2M Lithium Sulfate, 28% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.3, 0.2M Lithium Sulfate, 29% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.3, 0.2M Lithium Sulfate, 31% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.3, 0.2M Lithium Sulfate, 32% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.3, 0.2M Lithium Sulfate, 33% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH7.5, 0.2 M Lithium Sulfate, 30% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.5, 0.2 M Lithium Sulfate, 28% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.5, 0.2 M Lithium Sulfate, 29% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.5, 0.2 M Lithium Sulfate, 31% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH7.75, 0.2 M Lithium Sulfate, 33% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH8.0, 0.2 M Lithium Sulfate, 30% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH8.0, 0.2 M Lithium Sulfate, 28% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH8.0, 0.2 M Lithium Sulfate, 29% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH8.0, 0.2 M Lithium Sulfate, 31% PEG 3000	add new		Hampton_24_well
Jan 18, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	nothing	hanging drop	0.1 M Immidazole pH8.0, 0.2 M Lithium Sulfate, 33% PEG 3000	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.150 M, PEG 3000 30%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.150 M, PEG 3000 33%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.175 M, PEG 3000 31%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.175 M, PEG 3000 32%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.175 M, PEG 3000 33%	add new		Hampton_24_well
Feb 15, 2008	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M Immidazole pH=7.5, Lithium Sulfate 0.200 M, PEG 3000 30%		7.5	Hampton_24_well
Aug 04, 2011	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	20% PEG 3350, 0.2 M potassium sulfate, 5% glycerol
Nov 01, 2011	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	22% PEG 6000, and 0.1 M MES pH 5.0	Optimization screen	5.7
Dec 14, 2009	Chris Nelson	Washington University	WU_crystallization_protocol_1	crystal	hanging drop	0.1 M MES pH5.3, 1% glycerol, 20% PEG 6000