Purification

Target

IDP61359  

Purification ID

A20483  

Expression

M9 minimal (Dec 10, 2012)  

Experiment Date Start

Jan 24, 2013  

Experiment Date End

Feb 05, 2013  

Person

Gyorgy Babnigg  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  

Final Concentration (mg/mL)

254  

Final Volume (μL)

650  

Shipped To Lab Id

ANL  

Shipped Date

Feb 05, 2013  

Inserted

Jun 15, 2016