Purification

Target
CPX_63257_63258  
Purification ID
A20592  
Expression
LB + M9 + semet (Jun 05, 2016)  
Experiment Date Start
Jun 10, 2016  
Experiment Date End
Jun 12, 2016  
Person
Robert Jedrzejczak  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
7  
Final Volume (μL)
2500  
Shipped To Lab Id
ANL  
Shipped Date
Jun 12, 2016  
Inserted
Jul 27, 2016