Purification

Target
IDP90271  
Purification ID
W1  
Expression
LB (Oct 15, 2009)  
Experiment Date Start
Oct 17, 2009  
Experiment Date End
Oct 19, 2009  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_soluble_expression_1  
Status
success  
Description
NiNTA purification of soluble protein from 10 liter fermentor followed by FPLC purification on S200 superdex 10/60 column.  
Affinity Tag Status
uncleaved  
SeMet Status
native  
Final Buffer
25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
1.3  
Final Volume (μL)
7500  
Inserted
Oct 21, 2009