Purification

Target
IDP90271  
Purification ID
W3  
Expression
LB (Oct 15, 2009)  
Experiment Date Start
Oct 17, 2009  
Experiment Date End
Oct 19, 2009  
Person
Chris Nelson  
Lab
Washington University  
Protocol
WU_native_soluble_expression_1  
Status
success  
Description
NiNTA purification from 1 liter culture by 6HIS tag. No further cleanup.  
Affinity Tag Status
uncleaved  
SeMet Status
native  
Final Buffer
25 mM Hepes pH=7.4, 150 mM NaCl, 0.01% NaAzide  
Final Concentration (mg/mL)
15  
Final Volume (μL)
2000  
Inserted
Oct 21, 2009