Purification

Target
CPX_63257_63258  
Purification ID
A21066  
Expression
M9 minimal (Apr 15, 2015)  
Experiment Date Start
Jul 25, 2016  
Experiment Date End
Jul 27, 2016  
Person
Grazyna Joachimiak  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
196  
Final Volume (μL)
500  
Shipped To Lab Id
ANL  
Shipped Date
Jul 27, 2016  
Inserted
Dec 02, 2016