Purification

Target
IDP58950  
Purification ID
A21173  
Expression
M9 minimal + semet (Jan 20, 2016)  
Experiment Date Start
Jan 23, 2016  
Experiment Date End
Jan 25, 2016  
Person
Jerzy Osipiuk  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
1  
Final Volume (μL)
5000  
Shipped To Lab Id
ANL  
Shipped Date
Jan 25, 2016  
Inserted
Feb 16, 2017