Purification

Target
CPX_63257_63258  
Purification ID
A21464  
Expression
LB + 40 mM K2HPO4 (May 16, 2017)  
Experiment Date Start
Jun 15, 2017  
Experiment Date End
Jun 17, 2017  
Person
Grazyna Joachimiak  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
6  
Final Volume (μL)
25500  
Shipped To Lab Id
ANL  
Shipped Date
Jun 17, 2017  
Inserted
Jul 28, 2017