Purification

Target
CPX_06552_06553  
Purification ID
A22542  
Expression
M9 minimal (Mar 01, 2016)  
Experiment Date Start
Mar 06, 2016  
Experiment Date End
Mar 08, 2016  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
22  
Final Volume (μL)
444  
Shipped To Lab Id
ANL  
Shipped Date
Mar 08, 2016  
Inserted
Aug 20, 2018