Center for Structural Genomics of Infectious Diseases

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	pH
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Flouride .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M Ca Acetate, 20% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium dihydrogen Phosphate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium Formate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Thiocyanate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Chloride .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M Bis-Tris	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Ammonium Tartrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M Ca Acetate, 16% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M Ca Acetate, 20% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	0.1M Ca Acetate, 20% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium Chloride .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Ammonium Iodide .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Calcium chloride dihydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Iodide .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Sodium Acetate trihydrate .1 M	4.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Bis-Tris Propane .1 M	7
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M	5.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 20,000 10 %w/v Bis-Tris	5.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 10,000 20 % MES .1 M	6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 28 %v/v Calcium chloride dihydrate .2 M Sodium HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 5 %v/v ammonium sulfate 2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 6000 10 %w/v MPD 5 %v/v HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 10 %v/v polyethylene glycol 4000 20 %w/v Sodium HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	iso-Propanol 20 %v/v polyethylene glycol 4000 20 %w/v tri-Sodium Citrate dihydrate .1 M	5.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Succinic Acid pH 7.0 .8 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium dihydrogen Phosphate 2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M	5.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M HEPES	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium/Potassium Phosphate pH 8.2 1 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Formate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v tri-Sodium Citrate dihydrate	5.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M Sodium acetate trihydrate .1 M	4.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Ammonium Citrate pH 7.0 1 M Bis-Tris Propane .1 M	7
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 1.5 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Sulfate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Magnesium Acetate tetrahydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium dihydrogen Phosphate monohydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Ammonium hydrogen Citrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Sodium Acetate trihydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v tri-Lthium Citrate tetrahydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Sodium Citrate dihydrate .7 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 2000 30 %w/v Potassium Thiocyanate .1 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M	5.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 15 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M	5.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 25 %w/v Ammonium Sulfate .2 M Sodium acetate trihydrate .1 M	4.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Lithium Sulfate monohydrate .2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 30 %w/v Ammonium Sulfate .2 M Sodium Cacodylate .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	ammonium sulfate 2 M potassium sodium tartrate tetrahydrate .2 M tri-Sodium Citrate dihydrate .1 M	5.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 18 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 20 %w/v Magnesium Acetate tetrahydrate .2 M Sodium Cacodylate .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 15 %w/v Succinic Acid pH 7.0 .1 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 4000 30 %w/v Sodium Acetate trihydrate .2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M tri-Sodium Citrate dihydrate	5.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 8000 30 %w/v Sodium Acetate trihydrate .2 M Bis-Tris	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v sodium acetate trihydrate .1 M	4.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 400 2 %v/v Ammonium Sulfate 2 M Sodium HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 2000 28 %w/v Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 8 %w/v Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 10,000 20 %w/v Ethylene Glycol 8 %v/v HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M	5.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG 5KMME 25 %w/v Ammonium Sulfate .2 M HEPES	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 5000 20 %w/v Bis-Tris .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Pentaerythritol Ethoxylate (15/4 EO/OH) 30 %v/v ammonium sulfate .05 M Bis-Tris .05 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Jeffamine ED-2001 reagent pH 7.0 30 %v/v HEPES .1 M	7
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Chloride 3.2 M Bis-Tris Propane .1 M	7
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Ammonium Sulfate 2.5 M Sodium Acetate trihydrate .1 M	4.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	tri-Sodium Citrate dihydrate 1.4 M Sodium HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 2 M Sodium Acetate trihydrate .1 M	4.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	ammonium sulfate 1.6 M Sodium Chloride .1 M HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 3.5 M Sodium Acetate trihydrate .1 M	4.6
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 3.5 M Bis-Tris Propane .1 M	7
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium Formate 3.5 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Sodium/Potassium Phosphate 1 M Sodium/Potassium Phosphate	6.9
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v di-Sodium Tartrate dihydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium Acetate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Potassium dihydrogen Phosphate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v potassium sodium tartrate tetrahydrate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M HEPES .1 M	7.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 20 %w/v Magnesium Formate .2 M
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	PEG 5KMME 25 %w/v Calcium Chloride dihydrate .2 M Bis-Tris	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol 8000 9 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M	6.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Glycerol Anhydrous 12 %v/v ammonium sulfate 1.5 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	polyethylene glycol MME 2000 20 %w/v Trimethylamine N-Oxide .2 M Tris .1 M	8.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol 3350 25 %w/v Citric Acid .1 M	3.5
Mar 20, 2009	Olga Egorova	University of Calgary	Crystallization_protocol_Toronto	crystal	hanging drop	Polyethylene Glycol Monomethyl Ether 5000 30 %w/v ammonium sulfate .2 M MES .1 M	6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Flouride .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

0.1M Ca Acetate, 20% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium dihydrogen Phosphate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium Formate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Thiocyanate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Chloride .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M Bis-Tris

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Ammonium Tartrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

0.1M Ca Acetate, 16% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

0.1M Ca Acetate, 20% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

0.1M Ca Acetate, 20% PEG3350, 1mM Tyrphostin AG1478, 2mM ATP

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium Chloride .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Ammonium Iodide .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Calcium chloride dihydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Iodide .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Sodium Acetate trihydrate .1 M

4.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Bis-Tris Propane .1 M

7

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M

5.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 20,000 10 %w/v Bis-Tris

5.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 10,000 20 % MES .1 M

6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 28 %v/v Calcium chloride dihydrate .2 M Sodium HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 5 %v/v ammonium sulfate 2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 6000 10 %w/v MPD 5 %v/v HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 10 %v/v polyethylene glycol 4000 20 %w/v Sodium HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

iso-Propanol 20 %v/v polyethylene glycol 4000 20 %w/v tri-Sodium Citrate dihydrate .1 M

5.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v ammonium sulfate .2 M Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Succinic Acid pH 7.0 .8 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium dihydrogen Phosphate 2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M

5.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Ammonium Acetate .2 M HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Ammonium Sulfate 1.5 M HEPES

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium/Potassium Phosphate pH 8.2 1 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Formate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v tri-Sodium Citrate dihydrate

5.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M Sodium acetate trihydrate .1 M

4.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Ammonium Citrate pH 7.0 1 M Bis-Tris Propane .1 M

7

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 1.5 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Sulfate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Lithium Sulfate monohydrate .2 M HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Magnesium Acetate tetrahydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium dihydrogen Phosphate monohydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Ammonium hydrogen Citrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Sodium Acetate trihydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v tri-Lthium Citrate tetrahydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Sodium Citrate dihydrate .7 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 2000 30 %w/v Potassium Thiocyanate .1 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M

5.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 15 %w/v Ammonium Acetate .2 M tri-Sodium Citrate dihydrate .1 M

5.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 25 %w/v Ammonium Sulfate .2 M Sodium acetate trihydrate .1 M

4.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Lithium Sulfate monohydrate .2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 30 %w/v Ammonium Sulfate .2 M Sodium Cacodylate .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

ammonium sulfate 2 M potassium sodium tartrate tetrahydrate .2 M tri-Sodium Citrate dihydrate .1 M

5.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 18 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 20 %w/v Magnesium Acetate tetrahydrate .2 M Sodium Cacodylate .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 15 %w/v Succinic Acid pH 7.0 .1 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 4000 30 %w/v Sodium Acetate trihydrate .2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M tri-Sodium Citrate dihydrate

5.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 8000 30 %w/v Sodium Acetate trihydrate .2 M Bis-Tris

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Sodium Chloride .2 M HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v sodium acetate trihydrate .1 M

4.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 400 2 %v/v Ammonium Sulfate 2 M Sodium HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 2000 28 %w/v Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 8 %w/v Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 10,000 20 %w/v Ethylene Glycol 8 %v/v HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M

5.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG 5KMME 25 %w/v Ammonium Sulfate .2 M HEPES

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 5000 20 %w/v Bis-Tris .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Pentaerythritol Ethoxylate (15/4 EO/OH) 30 %v/v ammonium sulfate .05 M Bis-Tris .05 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Jeffamine ED-2001 reagent pH 7.0 30 %v/v HEPES .1 M

7

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Chloride 3.2 M Bis-Tris Propane .1 M

7

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Ammonium Sulfate 2.5 M Sodium Acetate trihydrate .1 M

4.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

tri-Sodium Citrate dihydrate 1.4 M Sodium HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 2 M Sodium Acetate trihydrate .1 M

4.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

ammonium sulfate 1.6 M Sodium Chloride .1 M HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 3.5 M Sodium Acetate trihydrate .1 M

4.6

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 3.5 M Bis-Tris Propane .1 M

7

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium Formate 3.5 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Sodium/Potassium Phosphate 1 M Sodium/Potassium Phosphate

6.9

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v di-Sodium Tartrate dihydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium Acetate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Potassium dihydrogen Phosphate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v potassium sodium tartrate tetrahydrate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M HEPES .1 M

7.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Magnesium Chloride hexahydrate .2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 20 %w/v Magnesium Formate .2 M

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

PEG 5KMME 25 %w/v Calcium Chloride dihydrate .2 M Bis-Tris

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol 8000 9 %w/v Calcium Acetate hydrate .2 M Sodium Cacodylate .1 M

6.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Glycerol Anhydrous 12 %v/v ammonium sulfate 1.5 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

polyethylene glycol MME 2000 20 %w/v Trimethylamine N-Oxide .2 M Tris .1 M

8.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol 3350 25 %w/v Citric Acid .1 M

3.5

Mar 20, 2009

Olga Egorova

University of Calgary

Crystallization_protocol_Toronto

crystal

hanging drop

Polyethylene Glycol Monomethyl Ether 5000 30 %w/v ammonium sulfate .2 M MES .1 M

6.5

Purification

Related Crystallization Drops