Purification

Target
CPX_97277_97378  
Purification ID
A23172  
Expression
LB + 40 mM K2HPO4 (Jun 17, 2019)  
Experiment Date Start
Jul 08, 2019  
Experiment Date End
Jul 10, 2019  
Person
Lucas Welk  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
failed  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleavage unsuccessful  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
1  
Final Volume (μL)
10000  
Shipped To Lab Id
ANL  
Shipped Date
Jul 10, 2019  
Inserted
Aug 06, 2019