Purification

Target

IDP97891  

Purification ID

A23322  

Expression

M9 minimal (Oct 15, 2019)  

Experiment Date Start

Nov 05, 2019  

Experiment Date End

Nov 06, 2019  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cut  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

68  

Final Volume (μL)

1700  

Shipped To Lab Id

ANL  

Shipped Date

Nov 06, 2019  

Inserted

Nov 15, 2019