Purification

Target

IDP51007  

Purification ID

W601  

Expression

LB (ampicillin) (Aug 02, 2020)  

Experiment Date Start

Jul 04, 2020  

Experiment Date End

 

Person

Chris Nelson  

Lab

Washington University  

Protocol

WU_standard_refolding  

Status

success  

Description

Arginine refolding followed by concentration, S200 sephadex sizing, then size exclusion on MonoQ in pH8.0 Tris-HCL in NaCl gradient to obtain pure homodimers.  

Affinity Tag Status

n/a  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM Hepes pH=7.4, 20 mM NaCl, 0.01% NaAzide  

Final Concentration (mg/mL)

12  

Final Volume (μL)

400  

Shipped To Lab Id

 

Shipped Date

 

Inserted

Aug 28, 2020