Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP52003
Purification ID: A23514
Expression: LB + 40 mM K2HPO4 (Aug 17, 2020)
Experiment Date Start: Sep 01, 2020
Experiment Date End: Sep 02, 2020
Person: Christine Tesar
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: PIG-I, SEC, IMAC-II::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: cleaved
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 38
Final Volume (μL): 450
Shipped To Lab Id: ANL
Shipped Date: Sep 03, 2020
Inserted: Sep 07, 2020

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	59	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	45	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	59	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	5	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	73	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	76	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	6	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	28	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	41	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	67	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	68	CrystalQuick standard profile, LBR round bottom plate
Apr 13, 2021	Christine Tesar	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	69	CrystalQuick standard profile, LBR round bottom plate