Purification

Target

IDP52003  

Purification ID

A23771  

Expression

LB + 40 mM K2HPO4 (Oct 31, 2021)  

Experiment Date Start

Nov 08, 2021  

Experiment Date End

Nov 09, 2021  

Person

Christine Tesar  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleaved  

SeMet Status

native  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

17  

Final Volume (μL)

380  

Shipped To Lab Id

ANL  

Shipped Date

Nov 10, 2021  

Inserted

Jan 31, 2022