Purification

Target
IDP97912  
Purification ID
A23941  
Expression
LB (Feb 25, 2022)  
Experiment Date Start
Mar 01, 2022  
Experiment Date End
Mar 03, 2022  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cut  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
36  
Final Volume (μL)
700  
Shipped To Lab Id
ANL  
Shipped Date
Mar 03, 2022  
Inserted
May 09, 2022