Purification

Target
IDP99023  
Purification ID
A23953  
Expression
LB (Feb 02, 2022)  
Experiment Date Start
May 09, 2022  
Experiment Date End
May 10, 2022  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cut  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
15  
Final Volume (μL)
300  
Shipped To Lab Id
ANL  
Shipped Date
May 10, 2022  
Inserted
May 16, 2022