Purification

Target
IDP00032  
Purification ID
A4434  
Expression
M9 minimal + semet (Jul 22, 2008)  
Experiment Date Start
Jul 24, 2008  
Experiment Date End
Jul 26, 2008  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
65  
Final Volume (μL)
500  
Shipped To Lab Id
ANL  
Shipped Date
Jul 26, 2008  
Inserted
Jul 29, 2011