Purification

Target
IDP00101  
Purification ID
A5256  
Expression
M9 minimal + semet (Mar 07, 2009)  
Experiment Date Start
Apr 17, 2009  
Experiment Date End
Apr 19, 2009  
Person
Rory Mulligan  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleavage unsuccessful  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
80  
Final Volume (μL)
1200  
Shipped To Lab Id
ANL  
Shipped Date
Apr 19, 2009  
Inserted
Jul 29, 2011