Purification

Target
IDP00070  
Purification ID
A3701  
Expression
M9 minimal (Jun 16, 2009)  
Experiment Date Start
Jul 01, 2009  
Experiment Date End
Jul 03, 2009  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleavage unsuccessful  
SeMet Status
native  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
82  
Final Volume (μL)
600  
Shipped To Lab Id
ANL  
Shipped Date
Jul 03, 2009  
Inserted
Jul 29, 2011