Purification

Target

IDP00060  

Purification ID

A4435  

Expression

M9 minimal + semet (Jul 22, 2008)  

Experiment Date Start

Jul 24, 2008  

Experiment Date End

Jul 26, 2008  

Person

Natalia Maltseva  

Lab

Argonne National Laboratory  

Protocol

standard purification_ANL  

Status

success  

Description

IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  

Affinity Tag Status

cleavage unsuccessful  

SeMet Status

semet  

Covalent Modification

 

Final Buffer

20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  

Final Concentration (mg/mL)

91  

Final Volume (μL)

450  

Shipped To Lab Id

ANL  

Shipped Date

Jul 26, 2008  

Inserted

Jul 29, 2011