Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00044
Purification ID: V275
Expression: SeMet (Jan 13, 2009)
Experiment Date Start: Jan 15, 2009
Experiment Date End: Jan 17, 2009
Person: Majka Klimecka
Lab: University of Virginia
Protocol: uva_expression_semet_1
Status: success
Description
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer
Final Concentration (mg/mL): 9.4
Final Volume (μL): 1000
Shipped To Lab Id
Shipped Date
Inserted: Jan 17, 2009

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Drop Precipitant Contents	pH	Plate Type
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1M HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1M HEPES, 0.2M Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1M HEPES, 0.2M Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1M HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1M HEPES, 15%w/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1Molal HEPES, 15%w/v PEG 3350, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 0.1Molal HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2Molal Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1Molal HEPES	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 13, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Feb 12, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	15%w/v PEG 3350, 0.1Molal HEPES, 0.2Molal Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1M HEPES, 15%w/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1M HEPES, 15%w/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.1M HEPES, 15%w/v PEG 3350, 0.2M Magnesium Chloride, 6-Hydrate	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Magnesium Chloride, 6-Hydrate, 0.1M HEPES, 15%w/v PEG 3350	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1M HEPES	7.5	Nextal hanging drop
Jan 31, 2009	Majka Klimecka	University of Virginia	UVA_default_crystallization	nothing	sitting drop	0.2M Magnesium Chloride, 6-Hydrate, 15%w/v PEG 3350, 0.1M HEPES	7.5	Nextal hanging drop