Purification

Target
IDP00117  
Purification ID
A3552  
Expression
M9 minimal + semet (Apr 02, 2008)  
Experiment Date Start
Apr 09, 2008  
Experiment Date End
Apr 11, 2008  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
7  
Final Volume (μL)
400  
Shipped To Lab Id
ANL  
Shipped Date
Apr 11, 2008  
Inserted
Aug 03, 2011