Purification

Target
IDP00139  
Purification ID
A3242  
Expression
M9 minimal + semet (Jul 10, 2008)  
Experiment Date Start
Jul 21, 2008  
Experiment Date End
Jul 23, 2008  
Person
Min Zhou  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC::TEV cleavage::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
cleavage unsuccessful  
SeMet Status
semet  
Final Buffer
50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM ß-mercaptoethanol, 250 mM Imidazole  
Final Concentration (mg/mL)
70  
Final Volume (μL)
600  
Shipped To Lab Id
ANL  
Shipped Date
Jul 23, 2008  
Inserted
Aug 03, 2011