Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP00328
Purification ID: A7067
Expression: M9 minimal (Mar 08, 2011)
Experiment Date Start: Mar 08, 2011
Experiment Date End: Mar 10, 2011
Person: Rory Mulligan
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: uncleaved
SeMet Status: native
Covalent Modification
Final Buffer: 50 mM HEPES pH 8.0, 500 mM NaCl, 5 % Glycerol, 10 mM Ã-mercaptoethanol, 250 mM Imidazole
Final Concentration (mg/mL): 42
Final Volume (μL): 700
Shipped To Lab Id: ANL
Shipped Date: Mar 10, 2011
Inserted: Nov 02, 2011

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
May 25, 2011	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	31	CrystalQuick standard profile, LBR round bottom plate
May 25, 2011	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	39	CrystalQuick standard profile, LBR round bottom plate
May 25, 2011	Rory Mulligan	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-1	74	CrystalQuick standard profile, LBR round bottom plate