Purification

Target
IDP01531  
Purification ID
A3538  
Expression
M9 minimal + semet (Jan 25, 2008)  
Experiment Date Start
Feb 10, 2008  
Experiment Date End
Feb 12, 2008  
Person
Minyi Gu  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC, IMAC-II::ÄKTAxpress using IMAC-I program::ÄKTAxpress IMAC-I program eluate (250 mM imidazole)  
Affinity Tag Status
cleaved  
SeMet Status
semet  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
2  
Final Volume (μL)
10000  
Shipped To Lab Id
ANL  
Shipped Date
Feb 12, 2008  
Inserted
Feb 27, 2012