Purification

Target
IDP01178  
Purification ID
A9221  
Expression
M9 minimal (Mar 14, 2012)  
Experiment Date Start
Apr 09, 2012  
Experiment Date End
Apr 10, 2012  
Person
Magdalena Makowska-Grzyska  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, SEC::SEC peak 1::concentration and buffer exchange (Amicon 3k or 10k)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
13  
Final Volume (μL)
1000  
Shipped To Lab Id
ANL  
Shipped Date
Apr 10, 2012  
Inserted
Apr 19, 2012