Purification

Target
IDP04364  
Purification ID
A9258  
Expression
M9 minimal (Mar 14, 2012)  
Experiment Date Start
Apr 11, 2012  
Experiment Date End
Apr 13, 2012  
Person
Natalia Maltseva  
Lab
Argonne National Laboratory  
Protocol
standard purification_ANL  
Status
success  
Description
IMAC-I, IMAC-II::ÄKTAxpress using IMAC-II program::ÄKTAxpress IMAC-II program flow-through (20 mM imidazole)  
Affinity Tag Status
na  
SeMet Status
native  
Final Buffer
20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT  
Final Concentration (mg/mL)
7  
Final Volume (μL)
25000  
Shipped To Lab Id
ANL  
Shipped Date
Apr 13, 2012  
Inserted
Apr 21, 2012