Center for Structural Genomics of Infectious Diseases

Purification

Target: IDP04364
Purification ID: A9256
Expression: M9 minimal (Mar 14, 2012)
Experiment Date Start: Apr 11, 2012
Experiment Date End: Apr 13, 2012
Person: Natalia Maltseva
Lab: Argonne National Laboratory
Protocol: standard purification_ANL
Status: success
Description: IMAC-I, IMAC-II::ÃKTAxpress IMAC-II program flow-through (20 mM imidazole)::concentration and buffer exchange (Amicon 3k or 10k)
Affinity Tag Status: na
SeMet Status: native
Covalent Modification
Final Buffer: 20 mM HEPES pH 8.0, 250 mM NaCl, 2 mM DTT
Final Concentration (mg/mL): 38
Final Volume (μL): 3
Shipped To Lab Id: ANL
Shipped Date: Apr 13, 2012
Inserted: Apr 21, 2012

Related Crystallization Drops

Experiment Date	Person	Lab	Protocol	Status	Method	Screen	Screen Component	Plate Type
Mar 19, 2014	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-PEG Screen	46	CrystalQuick standard profile, LBR round bottom plate
Aug 10, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-Minimal Screen	33	CrystalQuick standard profile, LBR round bottom plate
Aug 10, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	MCSG-3	94	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	36	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	31	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	36	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	63	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	69	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Index Screen	86	CrystalQuick standard profile, LBR round bottom plate
Aug 10, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-Minimal Screen	63	CrystalQuick standard profile, LBR round bottom plate
Apr 11, 2012	Natalia Maltseva	Argonne National Laboratory	standard crystallization_ANL	crystal	sitting drop	Pi-Minimal Screen	43	CrystalQuick standard profile, LBR round bottom plate