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Structure of IDP02733

Glucose-6-phosphate isomerase from Francisella tularensis.

Edit deposit information
CSGID target
IDP02733 
PDB Id
3LJK (NCBI MMDB
Authors
J.Osipiuk,N.Maltseva,J.Hasseman,W.F.Anderson,A.Joachimiak,Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Jerzy Osipiuk 
Responsible lab
Argonne National Laboratory 
Deposition Date
Jan 26, 2010 
Release Date
Mar 16, 2010 

Annotation

Description
Glucose-6-phosphate isomerase (also known as phosphoglucose isomerase (PGI)) is an enzyme that catalyzes the reversible aldose-ketose isomerization of glucose 6-phosphate (G6P) to fructose 6-phosphate (F6P). The reaction has direct roles in glycolysis and gluconeogenesis and, indirectly, other branches of carbohydrate metabolism such as the pentose phosphate pathway. PGI belongs to the aldose-ketose isomerase family of enzymes, all of which transfer a hydrogen atom between C1 and C2 of their respective substrates. PGIs use sugar substrates that exist in solution overwhelmingly in the hemiacetal or hemiketal ring form. PGIs also have a number of ‘moonlighting’ functions: it acts as an autocrine motility factor (AMF) and neuroleukin agent (NLK), a serine proteinase inhibitor and a differentiation and maturation mediator (DMM). It was found that GPI plays an essential role in the development of arthritis in mammals and may play roles in both cancer and autoimmunity. 
Functional assignment
Isomerase 

Ligands

Ligand code Name Ligand type
IPA
MES 2-(n-morpholino)-ethanesulfonic acid
GOL
CA
MSE modified residue

Structure information

Unit cell parameters

Space Group
P 32 2 1  
Unit Cell

a=115.06Å, b=115.06Å, c=84.45Å
α=90.00, β=90.00, γ=120.00 
Solvent content
52.8  
Matthews coefficient
2.61  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
32.30-1.48Å (1.52-1.48Å)  
Rall(%)
12.6 
Rwork(%)
12.4 (24.8) 
Rfree(%)
15.8 (30.4) 
Num. observed reflections
106466 (7227) 
Num. Rfree reflections
5323 (340) 
Completeness(%)
98.6 (91.3) 

Model parameters

Num Atoms
5477  
Num Waters
722  
Num Hetatoms
0  
Model mean isotropic B factor
11.580Å2  
RMSD bond length
0.019Å  
RMSD bond angle
1.629°  
Filename uploaded
idp02733.pdb (uploaded on Jan 26, 2010 4:56 PM)  
Inserted
Jan 26, 2010