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Structure of IDP90722

Crystal Structure of Biotin Carboxylase-ADP Complex from Campylobacter jejuni

Edit deposit information
CSGID target
IDP90722 
PDB Id
3OUZ (NCBI MMDB
Authors
N.Maltseva,Y.Kim,M.Makowska-Grzyska,R.Mulligan,L.Papazisi,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid) 
Responsible person
Natalia Maltseva 
Responsible lab
Argonne National Laboratory 
Deposition Date
Sep 15, 2010 
Release Date
Oct 13, 2010 

Annotation

Description
Biotin carboxylase, encoded by the accC gene, catalyzes the first step in fatty acid biosynthesis. It catalyzes the ATP-dependent transfer of carboxyl group from bicarbonate to biotin molecule of biotin carboxylase carrier protein (BCCP) . During second part of reaction transcarboxylase transfers the carboxyl group from carboxybiotinylated BCCP to acetyl-CoA to form malonyl-CoA and regenerate biotinylated BCCP. The formation of malonyl-CoA in turn controls the rate of fatty acid metabolism. Crystal structure of biotin carboxylase-ADP complex from Campylobacter jejuni was solved at 1.8 A resolution. The structure is of an alpha-beta fold and a dimer in the crystal. The structure is of an alpha-beta fold and protein molecules form dimer. Based on the structural peculiarities, the part of small domain (residues 133 - 202) swings back while interacting with adenosyl ligand to make compact patch. The binding region of biotin carboxylase is much conserved in different bacteria species. In accordance with what was found earlier in adenine-binding region Glu201-Leu204 and Ile157-Lys159 Leu204 is substituted by Ile204, ribose-binding region is formed by His209, Gln233, His236 and Ile437 is changed to Thr437, and His438 to Asn438. Phosphate groups of the ligand interact with glycine-rich group (residues 162-168) together with Lys116, Lys159 and Gln237 which interacts with the third phosphate group of the ligand.  
Functional assignment
 

Ligands

Ligand code Name Ligand type
ADP biological
TAR crystallization
MLT biological
GOL
MG
FMT
MSE modified residue

Structure information

Unit cell parameters

Space Group
P 21 21 21  
Unit Cell

a=89.86Å, b=100.67Å, c=147.70Å
α=90.00, β=90.00, γ=90.00 
Solvent content
62.98  
Matthews coefficient
3.32  

Refinement

Data for the highest resolution shell is in parentheses.
Resolution range
44.23-1.90Å (1.97-1.90Å)  
Rall(%)
15.2 
Rwork(%)
15.0 (2210.0) 
Rfree(%)
18.1 (2510.0) 
Num. observed reflections
110522 (10151) 
Num. Rfree reflections
5515 (516) 
Completeness(%)
99.6 (97.0) 

Model parameters

Num Atoms
8801  
Num Waters
906  
Num Hetatoms
178  
Model mean isotropic B factor
26.980Å2  
RMSD bond length
0.009Å  
RMSD bond angle
1.191°  
RMSD dihedral angle
14.59°
 
Filename uploaded
dep.pdb (uploaded on Sep 15, 2010 6:24 PM)  
Inserted
Sep 15, 2010