Clone

Target

IDP01457  

Clone Suffix

1

Sequence Comments

 

Experiment Start Date

Jun 06, 2008  

Experiment End Date

Jun 07, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG7  

Sequence Start

1  

Sequence End

580  

Reference Sequence Used

MKKKAPAKAS RVSPRVTTAE PAPIFIRWRF YLLLFFVLTA FCALVARVAY IQIIEPDNLI KEGDLRSIRA KTLQSARGII SDRNGEALAV SVPVEAVWAD PVAIYKEGGL VEKDRWYALA DVLGLDRQEM MKKIEDNRSR RFIYLQRQVS PAMAKYIREL KLAGIGLKEE SRRYYPAGEV SAHLVGVTGI DGHGLEGVER SFDSTLTGES GKSVTRKDKF GRVVENIALE EREEGKPIQL TIDQRLQAIA FRAVKQAMAD YRATSASAVM LDVKTGAVLA MVNAPSYNPN NRNDWQSFKM RNRVITDAFE PGSTVKPFVV LAALANGIAD KNTIIDTGDG TMQIGGSRVR DTSKVGKADL TLILKKSSNI GVAKLALEMP LEALLGMYSS VGLGEMSGLD LVGETIGIFP NRRRWSQFEI ATLSFGYGLA ITPIQLAHAY ATLGNHGKYQ PIHIIKGDQH GETRQVVDPK YVNQVLEMLE TVTQPGGTAT RAAVPGYRIA AKSGTSRKAI AGGYSDEYFA YTAGVAPVSD PRISLVVMVN EPQGDSYYGG AVAGPVFSEI MKGALQILNI APDENRFQNK  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

 

PCR Forward Primer

TACTTCCAATCCAATGCGATGAAGAAGAAAGCCCCAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATTTGTTTTGGAAGCGGTTTT  

Shipped To Lab

 

Shipped Date

 

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Aug 08, 2008