Clone

Target

IDP00017  

Clone Suffix

2 - The clone is available in BEIR but it is out of stock.

Sequence Comments

 

Experiment Start Date

Apr 21, 2008  

Experiment End Date

Apr 22, 2008  

Person

Jason Stam  

Lab

J. Craig Venter Institute  

Protocol ID

CSG-003_PCR Amplification and Ligation Independent Cloning (LIC)  

Status

success  

Vector

pMCSG19c  

Sequence Start

1  

Sequence End

397  

Reference Sequence Used

MFHRIEEALE DLKQGKVVIV CDDENRENEG DFIALAEYIT PETINFMITH GRGLVCVPIT EGYAERLQLE PMVSHNTDSH HTAFTVSIDH VSTTTGISAH ERATTIQQLL NPASKGADFN RPGHIFPLIA KEGGVLRRAG HTEAAVDLAQ LCGAEPAGVI CEIINEDGTM ARVPDLLQCA KQFDIKMITI EDLIAYRRHH ETLVTREVEI TLPTDFGTFQ AIGYSNSLDT KEHIALVKGD ISTGEPVLVR VHSECLTGDV FGSCRCDCGP QLHAALAQIE REGKGVLLYM RQEGRGIGLL NKLRAYKLQE EGFDTVEANE KLGFPADLRD YGIGAQILKD LGLQHLRLLT NNPRKIAGLQ GYDLTVTERV PLQMPAKEEN KTYLQTKVNK LGHLLNL  

Reference Sequence Variance

MATCH  

Confirmed Protein Sequence

MFHRIEEALE DLKQGKVVIV CDDENRENEG DFIALAEYIT PETINFMITH GRGLVCVPIT EGYAERLQLE PMVSHNTDSH HTAFTVSIDH VSTTTGISAH ERATTIQQLL NPASKGADFN RPGHIFPLIA KEGGVLRRAG HTEAAVDLAQ LCGAEPAGVI CEIINEDGTM ARVPDLLQCA KQFDIKMITI EDLIAYRRHH ETLVTREVEI TLPTDFGTFQ AIGYSNSLDT KEHIALVKGD ISTGEPVLVR VHSECLTGDV FGSCRCDCGP QLHAALAQIE REGKGVLLYM RQEGRGIGLL NKLRAYKLQE EGFDTVEANE KLGFPADLRD YGIGAQILKD LGLQHLRLLT NNPRKIAGLQ GYDLTVTERV PLQMPAKEEN KTYLQTKVNK LGHLLNL  

PCR Forward Primer

TACTTCCAATCCAATGCGATGTTTCATCGTATTGAAGAAGC  

PCR Backward Primer

TTATCCACTTCCAATGTTATAAGTTTAATAAATGTCCTAATT  

Shipped To Lab

UT  

Shipped Date

May 08, 2008  

Secondary Shipped To Lab

 

Secondary Shipped Date

 

Inserted

Sep 15, 2008